In modern terms, it won’t matter one bit because there’s a fluidity to what’s acceptable for people to be.
The issue would be how to handle a literal view of the old-school trans identity: “woman trapped in a man’s body”. I mean this stuff is all over the place, now, but you have the classic set-up for an existential crisis that’s 20+ years too late for whatever is going on right now.
Or at least that’s a risk. It doesn’t have to be that way.
For me? I play the Fable series. A woman who plays a straight male character because the relationships with men sounds like you’re hurting them in the middle of the act (it’s how you have kids in the game, and it’s fade-to-black scenes with some of the most horrific cringe lines in the gaming industry).
Anyway, my reasoning, when I thought about it, is I have no problem being what my skin is. If things truly aren’t gender-based, then “Hook or no hook, I’m Maui”. That level of security allows a former female and current male to go on with their life without 1 bit of care as to conversations about gender, sex, preference.
Basically, there doesn’t have to be a conflict to being 2 extremes in 1 person, if they can accept it and keep moving on.
The problem comes in with when you as a writer can’t handle that or when your character isn’t strong enough to deal with that. If either case is an issue, then both need to be the same, just for your sanity.
And then, of course, anyone who decides to take offense with the way you handle the conflict will also be an issue. Back to the example: I know who I am, but writing a character with my attitude about my own self? Yeah, that’s a risk of being told I’m insensitive.
Now I doubt that cleared up a single thing. Lmao